Eight new dinucleotide microsatellite loci in turkey (Meleagris gallopavo).
نویسندگان
چکیده
Source/Description: A phage library was constructed in dephosphorylated BamHI digested M13 from size-selected (220±1000 bp) turkey genomic DNA restricted with MboI. The library was screened with P-labeled (CA)16 and (GT)16 probes. Positive clones were sequenced with an automated DNA sequencer (ABI 373) at the Advanced Genetic Analysis Center, College of Veterinary Medicine, University of Minnesota. All sequences were queried against GenBank by BLAST searching. PCR primers were designed interactively with Primer v.3 (Whitehead Institute for Biomedical Research, http://www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi).
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ورودعنوان ژورنال:
- Animal genetics
دوره 31 2 شماره
صفحات -
تاریخ انتشار 2000